تعیین تجربی زبری سطح سلول سرطانی HT29 با استفاده از میکروسکوپ نیروی اتمی در جهت کاربرد در نانومنیپولیشن

نوع مقاله : مقاله کوتاه

نویسندگان

1 دانشگاه علم و صنعت ایران

2 دانشگاه اراک

چکیده

در این مقاله زبری سطح سلول سرطانی HT29 با استفاده از تحلیل تصاویر توپوگرافی به‌دست آمده توسط میکروسکوپ نیروی اتمی تعیین شده است. بر مبنای نتایج آنالیز زبری انجام‌شده و معادلات تئوری زبری رابینوویچ، شعاع متوسط برجستگی های سطح نمونه و سطح مبنا تعیین شده است. این مقادیر در روابط مدل دینامیک منیپولشن ذرات زبر وارد شده و مقادیر نیرو و زمان بحرانی منیپولیشن سلول‌ها شبیه‌سازی شده است. برمبنای نتایج، مد غلتشی مد غالب در شروع به حرکت سلول است و تحلیل برمبنای مدل رابینوویچ نسبت به مدل رامپ مقادیر نیرو و زمان بحرانی کمتری را پیش‌بینی می‌کند.

کلیدواژه‌ها


عنوان مقاله [English]

Experimental Determination of HT29 Cancerous Cell Surface Roughness by Atomic Force Microscopy to be Applied in Nanomanipulation

نویسندگان [English]

  • Moharam Habibnejad Korayem 1
  • Hedieh Badkoobehhezaveh 1
  • Moein Taheri 2
1 Iran University of Science and Technology
2 Arak University
چکیده [English]

In this paper, surface roughness of HT29 cancerous is determined using topography images obtained by Atimic Force Microscopy (AFM). The mean radiuses of cell and substrate surface asperities are determined, based on results of conducted roughness analysis and Rabinovich roughness theory. These values have been applied in the equations of rough particle nanomanipulation dynamic model and the amounts of critical force and time are simulated. Based on results, the rolling mode is the dominant dynamic mode at the onset of cells movement and the analysis based on rabinovich model predict lower amounts of critical force and time compared to Rumpf model based analysis.

کلیدواژه‌ها [English]

  • Surface Roughness
  • HT29 cancerous cell
  • Atomic Force Microscopy
  • Nanomanipulation
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