بررسی تجربی و تئوری مدول یانگ بافت سرطانی سینه(MCF-10) با استفاده از تیرک‌های مختلف میکروسکوپ نیروی اتمی

نوع مقاله : مقاله پژوهشی

نویسندگان

• معین طاهری ¹
• علی جباری ²
• زهراسادات اقدامی ²
• حامد فرجی ³
• نیما رحمانی ²

¹ مهندسی ساخت و تولید، دانشکده فنی و مهندسی، دانشگاه اراک، اراک.

² مهندسی ساخت و تولید، دانشکده فنی و مهندسی، دانشگاه اراک، اراک

³ گروه مهندسی مکانیک، دانشکده فنی و مهندسی، دانشگاه اراک ، اراک

10.22067/jacsm.2023.83806.1196

چکیده

امروزه میکروسکوپ نیروی اتمی کاربردهای مختلفی در ساخت قطعات ریز مقیاس و بررسی خواص مکانیکی آن‌ها دارد. مطالعه خواص مکانیکی بافت‌ها می‌تواند به‌عنوان عاملی مهم برای تشخیص زودهنگام سرطان و کمک به روش‌های جدید درمان‌های در نظر گرفته شود. روش‌های مختلفی برای تشخیص بافت‌های سرطانی وجود دارد که یکی از این راه‌ها بررسی مدول یانگ بافت است. یکی از جدیدترین روش‌ها برای استخراج مدول یانگ در بافت‌های بیولوژیکی استفاده از میکروسکوپ نیروی اتمی است. در این مطالعه، ابتدا از میکروسکوپ نیروی اتمی به‌منظور استخراج مدول یانگ بافت سرطانی سینه MCF-10 با استفاده از سه تیرک مختلف با هندسه‌های مستطیلی، وی-شکل و خنجری استفاده‌شده است. با توجه به تصاویر به‌دست‌آمده توسط میکروسکوپ نیروی اتمی، هندسه سلول کروی در نظر گرفته شد. نمودار نیرو- عمق نفوذ با میانگین‌گیری نتایج تجربی برای هرکدام از تیرک‌ها به‌طور مجزا رسم شد. در آخر مدول یانگ بافت سرطانی سینه برای سه تیرک با هندسه‌های مستطیلی، وی-شکل و خنجری با مدل تماسی هرتز استخراج‌شده است. با مقایسه نتایج تجربی و تئوری و با تغییر محدوده مدول یانگ فرضی برای هر سه هندسه تیرک‌ها، مشاهده شد که، استفاده از تیرک وی-شکل به دلیل اعمال نیروی کم‌تر به بافت، محدوده دقیق‌تری از مدول یانگ را پیش‌بینی می‌کند. مدول یانگ بافت سرطانی با استفاده از تیرک وی-‌شکل بین 1200 تا 1250 پاسکال در نظر گرفته شد.

کلیدواژه‌ها

• میکروسکوپ نیروی اتمی
• بافت سرطانی MCF-10
• تیرک مستطیلی
• تیرک خنجری
• تیرک وی-شکل

موضوعات

• مکانیک نانو ذرات

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عنوان مقاله [English]

Experimental and Theoretical Investigation of Young's Modulus of Breast Cancer Tissue (MCF-10) Using Different Cantilevers of Atomic Force Microscope

نویسندگان [English]

• Moein Taheri ¹
• Ali Jabbari ²
• Zahrasadat Eghdami ²
• hamed faraji ³
• Nima Rahmani ²

¹ Mechanical Engineering, Arak University, Arak, Iran.

² Department of Manufacturing, Faculty of Engineering, Arak University, Arak, Iran.

³ Department of Mechanical Engineering, Faculty of Engineering, Arak University, Arak

چکیده [English]

Today, the atomic force microscope has various applications in the manufacture of small-scale parts and the study of their mechanical properties. The study of mechanical properties of tissues can be considered as biomarkers for early detection of cancer and help in new treatments. There are diferent ways to detect cancerous tissues, and one of these ways is to check Young’s modulus of the tissue. One of the most recent methods for extracting Young's modulus in biological tissues is the use of atomic force microscopy. In this study, atomic force microscope was first used to extract Young's modulus of MCF-10 breast cancer tissue using 3 different cantilevers with rectangular, V-shaped and dagger geometries. The geometry of the cell was also assumed to be spherical according to the images obtained by atomic force microscopy. The force- indentation depth diagram was plotted by averaging the experimental results for each of the cantilevers separately. Finally, Young's modulus of breast cancer tissue for 3 cantilevers with rectangular, V-shaped and dagger geometries is extracted with Hertz contact model. By comparing the experimental and theoretical results and by changing the assumed range of Young's modulus for all 3 geometries of the cantilevers, it was observed that the use of V-shaped pole predicts a more accurate range of Young's modulus due to applying less force to the tissue. Young's modulus of breast cancer was considered between 1200 and 1250 (Pa) using V-shaped cantilevers.

کلیدواژه‌ها [English]

• Atomic force microscope
• MCF-10 cancer tissue
• Rectangular cantilevers
• Dagger cantilevers
• V-shaped cantilevers

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